Research Assistant I Lab

Job Summary

The Wagers laboratory studies the biology and function of adult tissue-specific stem cells, particularly those stem cells that form the skeletal muscle and blood. Current topics under investigation include regulation of stem cell function in normal tissues and under abnormal conditions such as aging and cancer, and methods for editing stem cell genomes using CRISPR/Cas9 for experimental and therapeutic purposes.

The lab is seeking highly motivated candidates to assist in ongoing experiments that include the development and application of novel CRISPR/Cas9 and adeno-associated virus (AAV) gene transfer technologies, mouse work (colony maintenance, husbandry, genotyping, physiological assessment, tissue harvest, etc.), cell culture, flow cytometry, immunostaining, and bioassays of skeletal muscle and hematopoietic cell function.

The assistant will also perform a variety of basic and general lab support and clerical tasks, including ordering and stocking of supplies, maintenance of equipment and preparation and storage of shared reagents. They will also be expected to participate in and present results for regular research group meetings and to contribute to the preparation and presentation of written and oral progress reports, as needed.

Work will be performed under the specific direction of the PI, research manager and/or senior lab members, although certain research-related tasks will be performed independently, within the guidelines of and subject to review by the PI and research staff.

Job-Specific Responsibilities

• Performs laboratory experiments utilizing AAVs and CRISPR/Cas9 genome editing complexes, gene engineered mouse models, cell culture, flow cytometry, immunostaining, microscopy, molecular biology and bioassays;
• Collects and safeguards project data; keeps detailed, accurate and up-to-date notes detailing experimental design and results;
• Under direction, processes, organizes and summarizes data, reporting experiment results in written form and in oral presentations, using a variety of scientific, word processing, spreadsheet and statistical software applications or program platforms;
• As a member of a project team, assists in the design and execution of laboratory experiments, techniques, and protocols;
• Performs routine logging and/or testing of samples; routine animal husbandry and experimentation, and genotyping
• May occasionally instruct others in basic laboratory techniques or procedures;
• Performs related laboratory maintenance such as maintaining and cleaning equipment, scheduling repairs, tracking inventory and ordering supplies.

Working Conditions and Physical Requirements

• May be required to lift, move and transport related laboratory equipment.
• Required to work with a variety of hazardous materials under approved safety protocols.
• Required to work with and handle animals.
• This position is based in Cambridge, MA

Basic Qualifications

At least one year related work experience (relevant course work may count towards experience)

Additional Qualifications and Skills

• Previous undergraduate or post-graduate lab experience, particularly with gene editing systems, animal models and tissue histology will be preferred.
• Demonstrated abilities in relevant laboratory techniques is also preferred.
• Exposure to applicable computer technologies, including specific software applications, is encouraged.
• Appropriate organizational and communications skills required.

Additional Information

• Appointment End Date: This is a one year grant funded term position with the possibility of extension based on availability of continued funding and satisfactory performance.
• Standard Hours/Schedule: 35 hours per week
• Visa Sponsorship Information: Harvard University is unable to provide visa sponsorship for this position

Pre-Employment Screening: Identity

Other Information: All formal offers will be made by FAS Human Resources

Work Format Details

• This position has been determined by school or unit leaders that all duties and responsibilities must be performed at a Harvard or Harvard-designated location.
• Certain visa types may limit work location.
• Individuals must meet work location sponsorship requirements prior to employment.

Pay and benefits

This position is salary grade level 052. Please visit  Harvard's Salary Ranges   to view the corresponding salary range and related information.

Benefits

• Harvard offers a comprehensive benefits package that is designed to support a healthy work-life balance and your physical, mental and financial wellbeing.
• Because here, you are what matters.
• Our benefits include, but are not limited to:

• Generous paid time off including parental leave
• Medical, dental, and vision health insurance coverage starting on day one

• Retirement plans with university contributions
• Wellbeing and mental health resources
• Support for families and caregivers

Professional development opportunities including tuition assistance and reimbursement