Research Associate I

About the role

Category: Science

Lab/Area: Bruneau Lab

About the Role

We are looking for candidates who are strongly motivated, work well in a team, and who could start July-August 2024. There is a potential for one or two publications within a two-year time period. Candidates with plans for graduate school, medical school or dental school are encouraged to apply.

What You Will Do

We are looking for a research associate whose primary role will focus on culturing induced pluripotent stem cell (iPSC) and performing molecular biology techniques to understand gene expression regulation during in vitro cardiac differentiation.

• iPSC culture: maintain and expand iPSC lines, including routine passaging, freezing, and thawing of cells
• Differentiation protocols: use differentiation protocols to generate cardiomyocytes, ensuring consistency and reproducibility, and checking differentiation efficiency by flow cytometry
• Molecular biology techniques: perform molecular biology techniques such as PCR, RT-qPCR, and western blotting to analyze gene expression and protein levels
• Use sequencing techniques such as RNA-seq, ChIP-seq, and ATAC-seq to investigate gene regulation and epigenetic changes in iPSC-derived cardiomyocytes
• Analyze experimental and sequencing data, and interpret results
• Updates records and log books and assists in maintaining lab records and inventory
• Assist in lab management functions including ordering reagents, organizing laboratory inventory

What You Will Need

• Associate's degree and 2+ years of relevant experience
• Excellent verbal and written communication skills
• Strong work ethic
• Strong organizational skills with attention to detail and the ability to multitask
• Excellent communication and interpersonal skills, with the ability to work effectively in a team setting.

What Is Preferred

• BA/BS in Biological sciences and at least 6 months practical experience in a research laboratory (preferred)
• iPSC or cell culture
• Flow cytometry
• Molecular biology skills such as PCR, western blotting, RT-qPCR
• Lab management (ordering, restocking, database maintenance)
• Additional Skills
• R or python programming (or equivalent)
• Experience with next-generation sequencing, e.g., RNA-seq, ChIP-seq
• CRISPR/Cas9 genome editing
• Proficiency in Excel, Word, ImageJ Fiji, Photoshop and Illustrator

Salary Range

$28.91/hr. ($60,147) - $33.32/hr. ($69,307)

Gladstone Perks & Benefits

• People -work with talented, committed, and supportive teammates within an organization that values each member of its community.
• A meaningful place to grow and learn -whether it's your professional skills or scientific knowledge, we have the resources and environment to advance either so you can better support Gladstone's mission to drive a new era of discovery in disease-oriented science and to mentor tomorrow's leaders in an inspiring and excellent environment.
• Healthy work/life balance -you are highly engaged and productive at work because you can have time to recharge and enjoy a vibrant life outside of work.
• Compensation -competitive salary. Title and salary will be commensurate with education and experience.
• Excellent benefits -generous medical, dental, vision, retirement plan, paid vacation, commuter benefits, access to free shuttle transportation.